Reservation & Fees

Rates below are effective July 1, 2026, and are subject to periodic evaluation and adjustment.

Rates listed assume that a user has been trained and is independent in instrument usage. An initial training session is provided at only the cost of the instrument time. A credit in the amount of the cost of the training session will be applied to the first independent user session. Imaging performed by center personnel will incur additional charges. Any consumables provided by the Imaging Center will be charged in addition to the hourly fees.  

*Daytime rates apply to usage from 9:30 a.m.-5:30 p.m., during which the center manager will be available for assistance and consultation. Off-hours usage (5:30 p.m.-9:30 a.m.) will be permitted at the discretion of the center manager and director for individuals who are trained and independent in their usage of specific instruments. Note that fees for imaging sessions that span daytime and off-hours will be the sum of those hours.

**The Arts and Sciences Imaging Center offers a variety of genomic and transcriptomic analysis solutions from 10x genomics. Below are descriptions of the most common and basic services, but note that there are many additional services and options available. Please schedule a consultation with center staff to discuss the most appropriate and cost-effective strategies for your project.

10x Genomics Chromium Single Cell 3' GEM-X v4 Reagents are used to partition single cells or nuclei and generate barcoded cDNA libraries for single cell RNA-sequencing analysis (scRNA-seq). Researchers provide viable single cell suspensions of the target cell populations. Imaging Center staff will process cells to create libraries ready for Illumina sequencing. The service includes:

• Evaluation, quantification, and viability assessment of input cells.
• Single cell capture of desired target number of cells (500-20,000).
• Generation and amplification of single cell cDNAs.
• Construction of Illumina sequencing-ready indexed cDNA libraries.
• Quality assessment and quantification of libraries.

Services DO NOT include sequencing or bioinformatic analysis. Projects must be discussed in advance with center staff and a project safety questionnaire completed and approved. Please schedule sample submission two weeks in advance to ensure availability of staff and reagents.

10x Genomics Flex v2 Single Cell RNA profiling is a whole transcriptome probe-based assay to interrogate fixed cells. The multiplex capture makes this assay significantly more affordable than the 3' assay as well as allowing work with stabilized fixed cells.10x genomics has probe sets available for human, mouse and rat with NHP expected soon. Researchers provide fixed single cell suspensions (see specific fixation protocol) of the target cell populations. We can also fix and prepare cells for you for an additional fee. Cells will be processed to create libraries ready for Illumina sequencing. The service includes:

• Evaluation and quantification of input cells.
• Single cell capture of desired target number of cells (500-20,000).
• Hybridization and amplification of whole transcriptome probes.
• Construction of Illumina sequencing-ready indexed probe libraries.
• Quality assessment and quantification of libraries.

Services DO NOT include sequencing or bioinformatic analysis. Projects must be discussed in advance with center staff and a project safety questionnaire completed and approved. Please schedule sample submission two weeks in advance to ensure availability of staff and reagents.

10x genomics Visium Spatial Transcriptomics Reagents capture mRNA or probes from frozen or FFPE sections onto 2 micron barcoded tiled grids (Visium HD) to generate libraries that retain the spatial information for each spot/tile. Researchers section fresh frozen tissue or FFPE tissue onto a standard charged microscope slide. Core staff will fix, stain, image, and process slides to create libraries ready for Illumina sequencing. The service includes:

• Visium slides.
• Fixation (fresh frozen) or deparaffinization (FFPE) and H&E staining of tissue on Visium slides.
• Imaging of stained tissue.
• Generation and amplification of cDNAs or captured probes.
• Construction of Illumina sequencing-ready indexed cDNA or probe libraries.
• Quality assessment and quantification of libraries.

Services DO NOT include sectioning, sequencing or bioinformatic analysis. For each tissue block, RNA quality assessment and tissue adhesion evaluation are highly recommended (inquire for details). Immunofluorescence processing and imaging may be substituted for H&E staining for and additional fee. Please discuss projects with center staff and schedule sample submission two weeks in advance to ensure availability of staff and reagents.

*Visium HD slides have two capture areas (for two tissue slices),two slides per set (~10,000,000 spots; 2um each)

The CGI Core also offers services for additional transcriptomics and epigenomics assays, including 10x genomics 5' Gene Expression and Immune Profiling Assays, 10x Genomics Multiomics (scRNA-seq + scATAC-seq) Assays and 10x Genomics Chromatin Accessibility (scATAC-seq). Please contact us for more information.