Established Analytical Methods

The Research Mass Spectrometry lab has recently undergone a substantial upgrade of its analytical equipment. Due to this, numerous methods have been in the process of development and validation for the new equipment. We will continuously expand this list as more methods are validated.

The laboratory's primary objective is to establish and offer standardized workflows for campus investigators.

If you need a particular assay, please don't hesitate to get in touch with us, and we will be able to tailor a workflow to meet your specific requirements.

Please feel free to contact us with any inquiries or concerns.

Proteomics Assays

Untargeted Proteomics – Data Dependent Acquisition (DDA)
Discovery-based approach where the most abundant ions are selected for fragmentation, enabling broad protein identification.

Untargeted Proteomics – Data Independent Acquisition (DIA)
Comprehensive proteome profiling by acquiring MS/MS data across predefined mass windows, improving quantification consistency.

Targeted Proteomics – Parallel Reaction Monitoring (PRM)
High-resolution, targeted method for quantifying specific proteins or peptides with enhanced selectivity and accuracy. Customized based on experimental needs.

Gel Band and Immunoprecipitation Identifications
Protein identification from gel-excised bands or enriched immunoprecipitated samples.

Untargeted Analysis of Post-Translational Modifications (PTMs)
Discovery workflows focused on identifying post-translational modifications such as phosphorylation, acetylation, ubiquitination, methylation and more.

PFAS Assays

PFAS 537.1
Validated LC-MS/MS assay following EPA Method 537.1 for detection of PFAS in drinking water.

PFAS 1633
Draft EPA Method 1633 for comprehensive PFAS analysis in water, soil, and tissue matrices.

PFAS from Biological Sources (In Vivo Methodologies)
Custom workflows for quantifying PFAS in serum, feces, liver, kidney, and skeletal muscle tissues.

Custom & Exploratory Assays

Custom Small Molecule Assays
Tailored LC-MS/MS methods developed to support investigator-driven research needs.

Determination of Unknown Compound Molecular Weights
Direct infusion and LC-MS/MS workflows for identifying the monoisotopic masses of unknown compounds.

Pharmaceuticals

Metformin
Validated, targeted LC-MS/MS assay for quantifying metformin in biological samples.

Cilostazol
Targeted LC-MS/MS assay for the quantification of cilostazol in biological matrices.

Sildenafil
Targeted LC-MS/MS assay for the quantification of sildenafil in biological matrices.

Nicotine Metabolites

Nicotine, Cotinine, trans 3-OH-cotinine
Quantitative LC-MS/MS analysis of nicotine and its primary metabolites in biological samples.

Plant-Derived Compounds

Plant Compounds – Alkaloids
Targeted or discovery-based workflows for analysis of plant-derived alkaloids. Method validated for vinblastine, anhydrovinblastine, and catharanthine, vindoline, tabersonine, ajamalicine.