Orientation for Users of Vertebrate Animals in DLAR (DLAR-TRG-13)
Training
DLAR Training Quick Links
DLAR Presentations and Workshops
IACUC Continuing Education Credits available with attendance at DLAR workshops.
- Please request/schedule for all Workshops and Trainings at least one week before scheduled date.
- WORKSHOPS WILL BE SCHEDULED BETWEEN 1-3 PM. Time may be adjusted to work with DLAR Staff Scheduling
- Workshops will be held in the DLAR Medical Center Facility
- Only procedures listed in the protocol will be trained.
For other inquiries, please contact the DLAR Training Group (Subject: DLAR Training Request).
Orientation for Users of Vertebrate Animals in DLAR (DLAR-TRG-13) Dates
The "Orientation for Users of Vertebrate Animals in DLAR" (DLAR-TRG-13) provides the investigator and their research staff with valuable information for navigating DLAR facilities easily and successfully.
Topics include:
About the Division of Laboratory Animal Resources at the University of Kentucky, Federal Regulations, IACUC & Protocol Considerations, Working with Animals in DLAR Facilities, Safety, Facility Access and Security, Animal Housing Systems, Humane Euthanasia, and DLAR Contacts.
The presentation lasts about 90 minutes, we recommend scheduling two hours in the event there are questions.
Scheduling
Orientation for Users of Vertebrate Animals in DLAR (DLAR-TRG-13) are the 1st and 3rd Fridays at 1:00 PM and the 2nd and 4th Tuesdays at 10:30 AM. Click on the hyperlink to schedule.
If the posted dates do not work with your lab schedule, please reach out to the DLAR Training Group to inquire about setting up a Personal/Lab session.
PDF version of the 2026 Orientation for Users of Vertebrate Animals in DLAR (DLAR-TRG-13) schedule.
DLAR Mouse Breeding Colony Management (DLAR-TRG-15)
To request the DLAR Mouse Breeding Colony Management (DLAR-TRG-15).
DLAR-TRG-15 Breeding Colony Management Handout (PDF, 77 pgs).
Handouts
DLAR-TRG-15 Mouse Breeding Colony Management (PDF, 58pgs)
DLAR-TRG-17E DLAR Designation-Flag Cards
DLAR-TRG-17I DLAR Pink Surgery Cards (PDF, 10pgs)
DLAR-TRG-17F Working in a Rodent Barrier Room (PDF, 41pgs)
DLAR-TRG-17H Capturing an Escaped Mouse
DLAR WSHP-01 DLAR Humane Rodent Euthanasia Using Carbon Dioxide (PDF, 32pgs)
DLAR-WSHP-02 Aseptic Surgery Techniques (PDF, 23pgs)
DLAR-WSHP-03 Post-Surgical Monitoring and Record Keeping (PDF, 36pgs)
DLAR-WSHP-04 Restraint & Handling for Mice and Rats
DLAR-WSHP-09 Injection Techniques for Mice and Rats (PDF, 28pgs)
DLAR-WSHP-10 Basic Suturing for Rodent Wound Closure
DLAR-WSHP-11 Using Rodent Anesthesia Machines (PDF, 27pgs)
Micro-Isolator Technique (PDF, 27pgs)
PPE: What Is It and Why Do I Have to Wear It? (PDF, 33pgs)
Hands-on Rodent Surgical Workshop
Our instructor for this workshop will be Dr. Cheryl Haughton, Senior Clinical Veterinarian.
Research staff will need to complete prerequisite training prior to enrollment in this rodent surgical workshop. Prerequisite training will include: Mouse and Rat Handling; Mouse and Rat Injection Techniques; Use of Rodent Anesthesia Machine; Principles of Aseptic Rodent Surgery; Suturing Basics for Rodents; and Post Surgical Monitoring. Forms of this training include the AALAS learning library, DLAR online or workshop presentations, demonstrated experience, and work with inanimate models.
Please contact Dr. Haughton to discuss prerequisites.
To schedule for this Workshop, please contact the DLAR Training Group (Subject: Hands-on Rodent Surgical Workshop)
Humane Techniques for Rodent Euthanasia using carbon Dioxide (DLAR-WSHP-01)
To schedule for DLAR-WSHP-01 Humane Techniques for Rodent Euthanasia Using Carbon Dioxide for further information and reservations for the Humane Techniques for Rodent Euthanasia Using Carbon Dioxide Seminar.
Rodent Euthanasia Guidelines and Recommendations Workshop Handout (PDF 32 pgs.)
Guide for Carbon Dioxide Euthanasia Steps (PDF 8 pgs.)
DLAR Aseptic Surgery Techniques (DLAR-WSHP-02)
This workshop will demonstrate the appropriate way to prep your surgical area and patient in an aseptic manner. Handouts will be offered for future reference as well. To schedule for DLAR-WSHP-02 Principles of Aseptic Surgery Techniques
DLAR-WSHP-02 Aseptic Surgery Techniques Handout (PDF 23 pgs.)
DLAR Post-Surgical Monitoring (DLAR-WSHP-03)
To schedule for DLAR-WSHP-03 Post Surgical Monitoring
Post-Surgical Monitoring and Records Handout (PDF, 36 pgs.)
DLAR Basic Handling of Mice and Rats & Other Common Procedures (DLAR-WSHP-04)
To schedule for DLAR-WSHP-04 Basic Handling of Mice and Rats
DLAR-WSHP-04 DLAR Basic Handling of Mice and Rats Handout (PDF 12 pgs.)
Injection Techniques, Restraint, & Handling for Mice and Rats (DLAR-WSHP-09)
This workshop covers various injection techniques for mice and rats. To schedule for DLAR-WSHP-09 Basic Handling of Mice and Rats
Please view the information:
Injection Techniques for Mice and Rats Handout (PDF 28 pgs.)
DLAR Basic Suturing (DLAR-WSHP-10)
To schedule for DLAR-WSHP-10 Basic Suturing for Rodents
Suturing Basics Handout (PDF 24 pgs)
DLAR Using the Rodent Anesthesia Machine (DLAR-WSHP-11)
Contact Glenn Florence and Kristin Fox DLAR Research Analyst for further information and reservations for Use of Rodent Anesthesia Machines Workshop.
Using Rodent Anesthesia Machines Handout (PDF, 27 pgs)
Waste Anesthesia Gas handout
Further Information on Research Species
- Common Mouse Research Models (pdf, 14pgs)
- Commonly Used Strains of Rats (pdf, 8pgs)
- Biology and Husbandry of Hamsters (pdf, 30pgs)
Seminar notes from the DLAR presentation The 3 "R"s plus One are available for viewing and download. If you wish to schedule a seminar for your group, please contact the DLAR Training Group.
Additional Training Available
A wide variety of training opportunities are available through the Division of Laboratory Animal Resources including Aseptic Surgery guidelines, restraint and blood collection, oral gavage and injection techniques. Our staff has experience in the care and handling of most common laboratory animal species and is only too happy to assist you and your staff. Inquires for group or individual sessions can be scheduled by contacting our DLAR Training Group (Subject: Request Additional Training) or veterinary staff. Technical assistance may be obtained from our research analyst, Glenn Florence.
In addition to our library, consisting of printed materials, CD's and DVD's, individual and group session training, technical services through our research analysts, and veterinary consultations, there are a number of self-paced training modules available on the American Association for Laboratory Animal Science (AALAS) Learning Library.
The University of Kentucky Environmental Health and Safety provides numerous online training modules in Biosafety and other areas that may be of benefit to our researchers and their staff. Please take a few minutes to review the list to see if the modules are applicable to your work.
Environmental Health and Safety
Institutional Biosafety Committee
Institutional Animal Care and Use Committee training
Should you need additional information on biosafety, we have included a link to the most current version of the Biosafety in Microbiological and Biomedical Laboratories (BMBL) published by the Centers for Disease Control for your convenience.
Occupational Health and Safety Information
An important part of working with laboratory animals is knowing the potential risks involved with certain species. As of 16 September 2020, The IACUC and Office of the Attending Veterinarian have discontinued giving credit for the Occupational Health and Safety Information on myUK Learning. According to IACUC Policy 106 the only accepted Occupational Health and Safety version will come from AALAS Learning Library. Select University of Kentucky from Institution List and hit continue. Use your linkblue ID and Password and hit Login. Click on Libraries, second on left under search courses. Click on animal Care and Use Courses, Select on LATG Courses: 2016 LATG Training Manual. Select LATG 07 Occupational Health and Safety.
*If you are not a UK employee/Student please contact DLAR Training Team for assistance*